Oxford Protein Production Facility UK
The Oxford Protein Production Facility UK is a structural proteomics facility in the Division of Structural Biology of the Department of Medicine, Oxford University, funded by the Medical Research Council and Biotechnology and Biological Sciences Research Council as a National Resource Centre for protein production and crystallization.
The OPPF-UK aims to promote and facilitate high throughput structural biology for the UK academic community. It is the first stage in a structural proteomics programme for the UK and represents an essential stepping stone toward the practical exploitation of the wealth of information coming from the human genome sequencing projects.
The OPPF-UK is located in the Research Complex at Harwell, adjacent to the Diamond Light Source.
©2010 Medical Research Council
We offer parallel vector construction and expression screening and access to high-throughput crystallization using 100nl sitting drops. Please go to the Services tab for details. To apply, please register on our website and then complete a proposal form available under the Services tab. The OPPF-UK is open to all UK academic groups and the use of our facilities is "free at the point of access".
For information about transnational access to this facility, please go to the P-CUBE website.
Axford D, Owen RL, Aishima J, Foadi J, Morgan AW, Robinson JI, Nettleship JE,Owens RJ, Moraes I, Fry EE, Grimes JM, Harlos K, Kotecha A, Ren J, Sutton G,Walter TS, Stuart DI, Evans G. In situ macromolecular crystallography using microbeams.Acta Crystallogr D Biol Crystallogr. 2012 May;68(Pt 5):592-600.
Wilke S, Groebe L, Maffenbeier V, Jäger V, Gossen M, Josewski J, Duda A, Polle L, Owens RJ, Wirth D, Heinz DW, van den Heuvel J, Büssow K. Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development. PLoS One. 2011;6(12):e27829. Epub 2011 Dec 9.
Green VL, Verma A, Owens RJ, Phillips SE, Carr SB. Structure of New Delhi metallo-β-lactamase 1 (NDM-1). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1160-4.
Bird LE. High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli. Methods. 2011 Sep;55(1):29-37.
Research at the Oxford Protein Production Facility is enabled primarily by grants from the Medical Research Council with additional support from the Biotechnology and Biological Sciences Research Council.
